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2) has been removed, and both of the IS26s in 10 independent

the investigation of ‚edomite‘ archaeology and scholarly discourse

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steroid side effects Does only one end of IS26 participate in the reaction?One possible way in which the targeting could be achieved is if Tnp26 preferentially binds to one end of IS26 and the recombination reaction occurs at that end of the IS. In the experiments using a plasmid containing a frameshift in the tnp26 gene of only one IS26 copy, it was possible to use unique features introduced during the generation of the frameshifts to explore this possibility by determining the origin of each IS26 (L and R in Fig.5) in each cointegrate. In R388::IS26 FS R, the unique SwaI site of IS26 (Fig.2) has been removed, and both of the IS26s in 10 independent cointegrates recovered from pRMH762 recombination with R388::IS26 FS R were amplified by PCR and digested with SwaI. steroid side effects

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